Flow-injection Mi Crodetermination of Heavy Metal Ions Using a C O M Packed with Immobilized Apoenzyme Beads
نویسنده
چکیده
A novel idea for biochemical microanalysis of heavy metal ions in combination with flow-injection techniques is proposed. The microdetermination of the cofactors based on an apoenzyme reactivation method is reviewed exemplifying the assays for heavy metal ions with use of several kinds of immobilized metalloenzymes as the recognition elements for each metal ion.
منابع مشابه
Citronellyl Butyrate Synthesis in Non-Conventional Media Using Packed-Bed Immobilized Candida Rugosa Lipase Reactor
The synthesis of citronellyl butyrate by direct esterification reaction catalyzed by immobilized lipase from Candida rugosa was studied in a continuous packed bed reactor using n-hexane as organic solvent. Parameters such as residence time, temperature, and pH were examined. The optimum conversion was obtained at a flow rate of 1 ml/min (residence time 8 min), temperature of 50 °C, and pH 7.5. ...
متن کاملBioaffinity Based Immobilization of Almond (Amygdalus communis) b-galactosidase on Con A-layered Calcium Alginate-cellulose Beads: Its Application in Lactose Hydrolysis in Batch and Continuous Mode
In this study, immobilization of partially purified almond (Amygdalus communis) β-galactosidase on Con A layered calcium alginate-cellulose beads was investigated. Immobilized β-galactosidase retained 72% of theinitial activity after crosslinking by glutaraldehyde. Both soluble and immobilized enzyme exhibited the samepH and temperature optima at pH 5.5 and 50ºC, respectively. Howev...
متن کاملInteraction of some heavy metal ions with single walled carbon nanotube
The interaction between some heavy metal ions such as of Pb(II), Cd(II) and Cu(II) ions from aqueous solution adsorbed by single walled carbon nanotube (SWCNTs) and carboxylate group functionalized single walled carbon nanotube (SWCNT-COOH) surfaces were studied by atomic absorption spectroscopy. The effect of contact time, pH, initial concentration of ion, ionic strength and temperature on the...
متن کاملInteraction of some heavy metal ions with single walled carbon nanotube
The interaction between some heavy metal ions such as of Pb(II), Cd(II) and Cu(II) ions from aqueous solution adsorbed by single walled carbon nanotube (SWCNTs) and carboxylate group functionalized single walled carbon nanotube (SWCNT-COOH) surfaces were studied by atomic absorption spectroscopy. The effect of contact time, pH, initial concentration of ion, ionic strength and temperature on the...
متن کاملEnzyme packed bed system for the on-line measurement of glucose, glutamate, and lactate in brain microdialysate.
Rapid measurement of glucose, glutamate, and lactate is important in understanding the dynamics of the energy balance of the brain. Glutamate is also the main excitatory neurotransmitter. A general immobilized enzyme-based flow injection assay system is described which uses oxidase and peroxidase enzymes to convert the analyte into an oxidized ferrocene species which is detected electrochemical...
متن کامل